Water clustering in a dehydrated zooglie “tibetan milk mushroom”

  • T. V. Krupskaya Chuiko Institute of Surface Chemistry NAS of Ukraine
  • Ja. Skubiszewska-Zieba Maria Curie-Sklodowska University
  • B. Charmas Maria Curie-Sklodowska University
  • M. D. Tsapko Taras Shevchenko Kyiv National University
  • V. V. Turov Chuiko Institute of Surface Chemistry NAS of Ukraine
Keywords: milk mushroom, water polyassociates, 1H NMR spectroscopy, DSC - thermograms


By the methods of DSC, NMR spectroscopy and thermal analysis, the state of water in partially dehydrated cell cultures of the Tibetan milk fungus was studied. It was found that when the water content in the samples is up to 0.8 g/g, all water is associated with cellular structures. Water is predominantly in a highly associated state, when there are 2-3 hydrogen bonds per molecule. In air at CH2O = 0.3 g/g, most of the water is part of clusters with a radius of R = 10 nm. With an increase in water concentration to 0.8 g/g, three maxima are present on the distribution curves at R = 1, 2, and 12 nm. The same maxima are also present when air is replaced by a chloroform medium. It is possible to differentiate the structure of various water clusters in the cellular systems of a Tibetan milk fungus using chloroform medium with the addition of trifluoroacetic acid. In this case, in the 1H NMR spectra there are four water signals related to clusters having different dissolving powers with respect to acid. About half of the bound water is part of acid-insoluble clusters. Two endothermic peaks are recorded on thermograms of thawing of a pure dean. One at T = -28 °C, which coincides with the passport melting point of the dea, and the second at T = -8 °C. Probably, part of the decane is able to maintain crystallinity at a temperature significantly higher than the melting temperature of the bulk of the substance. On the DSC curves of thawing of a sample of milk fungus biomaterial containing equal amounts of adsorbed water and decane, several endothermic maxima are observed. Considering that at the chosen concentration, the peak of water defrosting is not observed, the most intense peaks (T = -28 and T = -25 °C) should be attributed to the process of dean melting. Peaks of lower intensity can also be associated with the melting of various forms of crystalline decane stabilized by the surface of the biomaterial


Oleskin A.V. Social behaviour of microbial populations. J. Basic Microbiol. 1994. 34(6): 425. https://doi.org/10.1002/jobm.3620340608

Rossello-Mora R. A., Wagner M., Amman R., Schile K.-H. The Abundance of Zoogloea ramigera in Sewage Treatment Plants. Applied and Enviromental Microbiology. 1995. 61(2): 702.

Fridman B.A., Dugan P.R., Pfuster R.M., Remsen C.C. Fine Structure and Composition of the Zoogloeal Matrix Surrounding Zoogloea ramigera. Journal of Bacteriology. 1968. 96(6): 2144.

Khachatryan A.A., Erofeeva L.M., Kutvitskaya S.A. The role of neuroglia in the functioning of the nervous system. The successes of modern science. 2014. 6: 66. [in Ukrainain].

Costerton J.W. Microbial interactions in biofilms. Beijerinck Centennial. Microbial Physiology and Gene Regulation: Emerging Principles and Applications. Book of Abstracts /Ed. W.A. Scheffers, J.P. van Dijken. Delft. Delft. Univ. Press: 1995: 20.

Afanasyeva O.V. Milk Mushroom: Tibetan riddle: The latest treatment methods; Unique cosmetic recipes. AST: Astrel-SPb. 2006: 127. [in Ukrainain].

Mitrofanova T.A. Tibetan mushroom: Treatment of allergies, restoration of intestinal microflora. IH: "Ves", 2005: 128.

Gun'ko V.M., Turov V.V., Gorbik P.P. Water at the interface. Кyiv: Naukova dumka. 2009: 694. [in Ukrainain].

Gun'ko V.M., Turov V.V. Nuclear Magnetic Resonance Studies of Interfacial Phenomena. New York: Taylor & Francis, 2013: 1070. https://doi.org/10.1201/b14202

Turov V.V., Gun'ko V.M. Clustered water and its application. Kyiv: Naukova dumka. 2011: 316. [in Ukrainain].

Gun'ko V.M., Turov V.V., Bogatyrev V.M. et. al Unusual Properties of Water at Hydrophilic/Hydrophobic Interfaces. Adv. Colloid Interface Sci. 2005. 118: 125. https://doi.org/10.1016/j.cis.2005.07.003

Aksnes D.W., Kimtys L. Characterization of mesoporous solids by 1H NMR. Solid State Nuclear Magnetic Resonance. 2004. 25:146. https://doi.org/10.1016/j.ssnmr.2003.03.001

Petrov O.V., Furo I. NMR cryoporometry: Principles, application and potential. Progr. NMR. 2009. 54: 97. https://doi.org/10.1016/j.pnmrs.2008.06.001

Frolov Yu.G. The course of colloid chemistry. Surface phenomena and disperse systems. Moscow: Chemistry. 1982: 400. [in Ukrainain].

Derome A.E. Modern NMR Tecnoque for Chrmistry Research. Pergamon Press. Oxford New York Beijing Frankfurte Sao Paulo Sodney Tokyo Toronto. 390

Abragam, A. The Principles of Nuclear Magnetism; Oxford University Press: Oxford. UK. 1961. https://doi.org/10.1063/1.3057238

Franks F. Biophysics and biochemistry at low temperature. Cambridge: University Press. 1985: 210.

Höhne G., Hemminger W., Flammersheim H.-J. Differential Scanning Calorimetry: An Introduction for Practitioners. Springer-Verlag. 1996: 222. https://doi.org/10.1007/978-3-662-03302-9

Lee J., Kaletunç G. Evaluation of the Heat Inactivation of Escherichia coli and Lactobacillus plantarum by Differential Scanning Calorimetry. Appl Environ Microbiol. 2002. 68(11): 5379. https://doi.org/10.1128/AEM.68.11.5379-5386.2002

Mohacsi-Farkas, C., J. Farkas, L. Meszaros, O. Reichart, E. Andrassy. Thermal denaturation of bacterial cells examined by differential scanning calorimetry. J. Therm. Anal. Calor. 1999. 57: 409. https://doi.org/10.1023/A:1010139204401

Gun'ko V.M., Turov V.V., Krupska T.V. et. al Interfacial behavior of silicone oils interacting with nanosilica and silica gels. J. Colloid and Interface Science. 2013. 394: 467. https://doi.org/10.1016/j.jcis.2012.12.026

Turov V.V., Gun'ko V.M., Zarko V.I., et. al Interfacial Behavior of n-Decane Bound to Weakly Hydrated Silica Gel and Nanosilica over a Broad Temperature Range. Langmuir. 2013. 29: 4303. https://doi.org/10.1021/la400392h

How to Cite
Krupskaya, T. V., Skubiszewska-Zieba, J., Charmas, B., Tsapko, M. D., & Turov, V. V. (2019). Water clustering in a dehydrated zooglie “tibetan milk mushroom”. Surface, (11(26), 542-555. https://doi.org/10.15407/Surface.2019.11.542
Medical and biological problems of surface