Non-isothermal decomposition of biomolecules studied by means of temperature-programmed desorption mass spectrometry

  • V. A. Pokrovskiy Інститут хімії поверхні НАН України
  • N. P. Galagan Інститут хімії поверхні НАН України
  • T. V. Kulik Інститут хімії поверхні НАН України

Анотація

Temperature-programmed desorption mass spectrometry (TPD MS) is discussed with reference to studies of modified pyrogenic oxides. Simple theoretical models are presented in order to allow an approximate analytical treatment of TPD MS data. Rate constants for first- second- and third-order reactions have been obtained. Products of thermal decomposition of carbonized surface layer has been observed and identified. The different pathways of uni- and bimolecular desorption were investigated and rate constants of reactions has been derived from TPD MS measurements.

Adsorption and thermal stability of several polyketides has been studied by TPD MS in condensed and adsorbed states. Mass spectrometric analysis allowed observing correlation between chemical structure of morine and venorutone molecules and mechanism of thermolysis. Decomposition of carbon carcass of chromone, the bensanelled y-pyrone in particular, resulted in formation of fragments with mass numbers 91, 95, 108, 111 m/z. Decomposition occurred in three stages with maxima at 200, 280, 380°C for morine and 240, 320, 450°C for venorutone. Presence of carbohydrate residue (ramnose, glucose) in venorutone molecule caused two additional stages of thermolysis in comparison with morine, maxima being located at 220 and 280°C. In this case, different to previous one, the substituted y-pyrone has been produced being characterized by mass numbers 72, 97, 102, 126 m/z. Comparison of adsorption parameters for venorutone and carbohydrates has shown that carbohydrate residue played main role in adsorption of venorutone molecules on ultrafine silica surface. The ring C of venorutone molecule stayed free in adsorption process which attested the fact that hydroxyl group in position 4' responsible for biological activity of flavonoids, did not participate in adsorption. It allows the ring C preserve its antioxidant, membrane- and capillary-stabilizing activity in bound state.

 

Посилання

Chuiko A.A., Nazarenko V.A., Pokrovskiy V.A. Desorption mass spectrometry and its applications in surface chemistry // Visnyk AS USSR, - 1989. - N. 10. - P. 31 - 41.

The solid state thermal degradation of aspirin / G.V, Guchup, K.S. Alexander, D.        Dollimore et al. // Proc. 19th North Amer. Thermal Analysis Society Conf. (Ed. I R. Harrison) (Toledo, May 20 - 25, 1990). - P. 549-555.

Guchup G.V., Alexander K.S., Dollimore D. The Use of Thermal Analysis and Mass Spectrometry to Study the Solid State Behavior in Pharmaceutical Tablet Mixtures // Thermochimica Acta. - 1992. - V. 196. - P. 267-278.

The Determination of Cholesterol, Calcium Carbonate and Calcium Oxalate in Gallstones by Thermogravimetry / K.S. Alexander, D. Dollimore, J.G. Dunn et al. / Thermochimica Acta. - 1993. - V. 215. - P. 171-181.

The Influence of Pectin and Bile Salt/Lecithin/Cholesterol System on the Particle Size of Calcium Carbonate Suspensions / J.D. Skelton, K.S. Alexander, D. Dollimore et al. // S.T.P. Pharma. Sciences. - 1993. - V. 3,- P. 153-162.

Dollimore D., Martin A., Pinkerton A.A. A Study of the Thermal Decomposition of Biguadinium Diperchlorate Using Thermogravimetry and Mass Spectroscopy // Thermochimica Acta. - 1996. -V. 285. - P. 109-117.

Dollimore D., Lerdkanchanaporn S., Alexander K.S. The Use of the Harcourt and Esson relationships in Interpreting the Kinetics of Rising Temperature Solid State Decompositions and Its Application to Pharmaceutical Formulations // Thermochimica Acta. - 1996. - V 290. - P. 139-147.

Dollimore D. Thermal analysis // Analytical chemistry. - 1996,- V. 68, N 12. - P. 63R-71R.        

Pokrovskiy V.A.,Ustyuzhanin P.F. Model of activated state in cyancobalamine decomposition//Teoret. i Eksperim. Khimia. - 1988,-V. 24, N 6. - P.751-753.

Pokrovskiy V.A., Galagan N.P., Dembnovetskaya L.I. Mass spectrometry of cyanocobalamine, glucose and raffinose in the solid state and on a disperse oxide surface // Rapid Communications in Mass Spectrometry. - 1995. - V. 9. - P. 585-587.

Pokrovskiy V. A. Temperature-programmed mass spectrometry of biomolecules in surface chemistry studies// Rapid Communications in Mass Spectrometry. - 1995. - V. 9. - P. 588-591.

Thermal destruction of amino propyl-silica surface layer / E.M. Pakhlov, E.F.         Voronin, V.A. Pokrovskiy, A.A. Chuiko // Zhurnal prikladnoy khimii. - 1996. - V. 69, N3-P. 423-426.

Organosiloxane and monoethanolamine transformations on fumed silica and silica- alumina surfaces / A.A. Chuiko, G.Ya. Guba, V.A. Pokrovskiy, M.I. Terez // Colloids and surfaces. - 1996. - N 106,- P. 89 - 95.

Kulik T.V., Galagan N.P., Pokrovskiy V.A. Mass spectrometric investigation of thermolysis of sugars // Farmatsevtychny zhurnal. - 1997. - N 2. - P. 76 - 79.

Mass spectrometric studies on cyancobalamine on disperse oxide surface / V.A. Pokrovskiy, T V. Kulik, N.P. Galagan, A.A. Chuiko // Farmatsevtychny zhurnal. - 1997. -N 5. - P. 52-57.

Pokrovskiy V.A. Temperature-programmed desorption mass spectrometry (TPD MS) of dispersed oxides// Adsorption Science & Technology. - 1997. - V.M, N 5. - P. 301- 317.

Temperature-programmed desorption mass spectrometry of carbonized silica surface / V. A. Pokrovskiy, R. Leboda, V. V. Turov et al. // 1998,- Carbon.- N 17. - P. 1039­1047.

Gunko V.V., Leboda R., Pokrovskiy V.A. Pathways for decomposition of phenylethanol bound to silica surface. Polish J. Chem. 1999,- V. 73,- P. 1345-1356.

Kulik T.V., Galagan N.P., Pogoreliy V.K., Pokrovskiy V.A. TPD MS studies of several polyketides in solid state and adsorbed on silica surface // 15th International Mass Spectrometry Conference, Barcelona, Aug. 28-Sept. 1, 2000; Book Abstr P. 105.

Pokrovskiy V. A. Temperature-programmed desorption mass spectrometry // Journal of Themal Analysis and Calorimetry. - 2000. - V. 62,- P. 407-415.

Опубліковано
2001-06-10
Як цитувати
Pokrovskiy, V. A., Galagan, N. P., & Kulik, T. V. (2001). Non-isothermal decomposition of biomolecules studied by means of temperature-programmed desorption mass spectrometry. Поверхня, (4-6), 332-347. вилучено із http://surfacezbir.com.ua/index.php/surface/article/view/71