Studying the structure of nanodiamnds and their influence on the microvispensity of rat erythrocyte membranes by the method of spin probes

  • N. T. Kartel Chuyko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • L. V. Ivanov Chuyko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • A. N. Lyapunov Institute of Monocrystals of the National Academy of Sciences of Ukraine
  • O. A. Nardid Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine
  • O. V. Scherbak Kharkov Veterinary Academy
  • O. A. Gurova Nikolaev Institute of Inorganic Chemistry
  • A. V. Ototrub Novosibirsk State University
Keywords: spin probe, detonation nanodiamonds, electron paramagnetic resonance, paramagnetic centers, free radical processes, membrane microviscosity, bioavailability


Some features of the NDA structure, antioxidant properties, and the effect of NDA nanoparticles on the microviscosity of rat erythrocyte membranes were studied using the EPR method and spin probes. It is shown that when the concentration of DND in a water suspension equal to 1 mg/ml, partial agglomeration of DND particles occurs, which disappears when the suspension is diluted. The EPR spectra (recovery of the TEMPOL spin probe) revealed a change in the antioxidant activity of the studied DND with time, which allows the use of the EPR method for rapid testing of DND samples from different manufacturers. The calculation of the parameters of the EPR spectra showed that the presence of 25 µg/ml of DND in a suspension of red blood cells does not affect the correlation time of the probe in the membrane within the experimental error, and consequently, the microviscosity of the red blood cell membranes. The introduction of DND into a suspension of erythrocytes at a concentration of 25 μg/ml does not lead to significant changes in the microviscosity of erythrocyte membranes, which indicates a low cytotoxicity of DND at these concentrations.



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How to Cite
Kartel, N. T., Ivanov, L. V., Lyapunov, A. N., Nardid, O. A., Scherbak, O. V., Gurova, O. A., & Ototrub, A. V. (2019). Studying the structure of nanodiamnds and their influence on the microvispensity of rat erythrocyte membranes by the method of spin probes. Surface, (10(25), 286-297.
Medical and biological problems of surface